604 Harmony Lane
Pleasantville, CA 94588
Dynamic, forward-looking team player with 5 years’ experience, both tertiary and work experience related, in biotechnology. Combines extensive technical, analytical, and interpretive skills with practical field experience. Possess strong interpersonal skills: experience working with multi-discipline research/scientific teams and professionals. Proficient in English, Hebrew, and French. Areas of expertise include:
· GC-MS & LC-MS Analysis
· Water Quality Guidelines
· Solvent Extraction Methods
· Multivariate & Univariate Statistics
· Analysis of Biological Molecules
· Ecological Risk Assessment Principals & Methods
· Traditional & Advance Techniques in Microbiology & Molecular Biology
· Chemistry Microbiology Of Pollution Associated With Mining
Ph.D., Environmental Biotechnology
1999 to 2003
UNIVERSITY OF NEW YORK, New York
· The use of microbial phospholipid fatty acid profiling to measure the impact of acid rock drainage on microbial communities in sediments.
· Performed analysis, identification and quantification of microbial biochemical markers such as phospholipids, PHAs and ubiquinones using GC-MS and LC-MS.
· Conducted physio-chemical and microbiological analysis of polluted sediments and water samples (ie. Heavy metals analysis, BIOLOG analysis, viable count, microbial morphology, 16s rRNA analysis etc.).
· Carried out multivariate statistical analysis to correlate physio-chemical parameters with biological ones.
1996 to 1999
UNIVERSITY OF NEW YORK, New York
· Course included: Biochemistry, microbiology, immunology, and molecular biology.
Course provided professional qualifications in biological sciences with an emphasis on DNA technology and its applications, combined with a firm basis in the industrial aspects of biotechnology.
· Appointed as Project Leader for the establishment of a quality control plan for a pharmaceutical plant project, supervising up to 10 students and assisting team members.
· Acknowledged for strong skills in statistics, biochemistry and molecular biology, assisting fellow students in problem diagnosis and troubleshooting.
PROFESSIONAL EXPERIENCE & TRAINING
Scientific Officer – Microbiology Department, Environmental Division
1999 to 2003
ANSTO (AMERICAN NUCLEAR SCIENCE & TECHNOLOGY ORGANISATION), New Jersey
Team member of waste management project responsible for the evaluation of microbial molecular indicators for the measurement of ecological impact at a national research institute for environmental risk assessment.
· Research results on comparisons between polluted and unpolluted sites accepted for publication in the prestigious international scientific magazine “The Journal of Microbial Ecology”, with two other collaborative publications anticipated.
· Implemented quality control parameters in order to standardize and streamline the administration and analysis of experiments.
· Key player in the integration of team members’ research through statistical analysis, contributing significantly to the scientific knowledge of the organization.
· Collaborated with team members in determining the relationships between environmental stressors (heavy metals, pH, flow etc.) and microbial communities’ biomass and diversity.
· Trained team members on the various procedures for using statistical programs; enlisted as technical specialist due to high level of technical skills.
· Conducted an in-depth analysis on the various sediments to determine the ecological impact of acid mine drainage pollution on microbial communities through organic chemistry, analytical chemistry, biochemistry, microbiology, and analysis of vast amounts of data using multivariate statistics.
· Collaborated with project staff on the application of microbial bioindicators to determine ecological impact and recovery.
· Performed a number of projects and tasks unsupervised due to consistently solid performance. Projects included:
· Analysis of microbial phospholipid biomarkers using GC-MS and statistically analyzing the data.
· Analysis of microbial respiratory ubiquinone biomarkers using LC-MS.
· Analysis of microbial communities using carbon utilization assay (BIOLOG).
· Analysis of microbes using traditional microbiological methods.
· Assisted senior scientists in the evaluation and comparison of methods for assessment of environmental microbial communities.
Research Assistant (Work Experience)
DNA LABS – NEW YORK IVF, New York
Worked in a developing capacity as a research assistant with increased responsibility for paternity tests, DNA fingerprinting, Southern Blotting, PCR analysis, and individual projects using gel electrophoresis.
· Wrote and submitted report for special in-house project to establish the relation between microdeletions in the AZF regions of chromosome Y and male infertility.
· Collaborated with chief scientists in the preparation and execution of paternity tests, including preparation of the gel for electrophoresis, preparation of samples, and amplification with PCR.
Research Assistant (Scholarship Program)
CSIRO – FOOD SCIENCE NEW JERSEY, New Jersey
Performed a variety of research and analysis assignments in the field of food microbiology using independent judgment and diagnosis, including testing different batches of media with different inocula, assessing results, media making, resuscitation of lyophilized cultures, serial dilutions, and spread plating.
· Independently conducted several international collaborative studies in methods and media for enumeration of yeasts and moulds in foods.
· Played a key role in the optimization of international standard quality control techniques in the field of food microbiology.
· Wrote and submitted report for international collaborative study aimed at setting international quality control standards for specialized media.
ANALYTICAL CHEMISTRY TECHNIQUES
· Solvent Extractions
· UV Absorption Spec.
· Particle Measurement Analysis
· Solid Phase & Column Chromatography, TLC
· Biosynthesis & Chemical Structures of Fatty Acids
· Liquid Chromatography - Mass Spectrometer Analysis, HPLC
· HP4500 Inductively Coupled Plasma - Mass Spectrometer (ICP-MS)
· Microwave Assisted Acid Digestion (MLS1200 Mega laboratory microwave digester)
· Vista Simultaneous Inductively Coupled Plasma Atomic Emission Spectrometer (ICP-AES)
· CNS2000 – for rapid determination of Carbon, Nitrogen and Sulphur of mineral and environmental samples
· Identification & Quantification of Compounds using Gas Chromatography - Mass Spectrometer Analysis (extensive knowledge, 4 years experience using various instruments i.e. HP 5890 series II coupled to VG TRIO, Varian Saturn GC-MS etc)
STATISTICAL ANALYSIS – MULTIVARIATE & UNIVARIATE TECHNIQUES
· Student T-tests
· Covariant Analysis
· Regression Analysis
· Redundancy Analysis (RDA)
· Hierarchical Cluster Analysis
· Principle Component Analysis (PCA)
· Canonical Correspondence Analysis (CCA)
· Non-Parametric Multidimensional Scaling (nMDS) Ordination
MOLECULAR BIOLOGY & MICROBIOLOGY TECHNIQUES
· Paternity Tests
· Southern Blotting
· Bioreactor Operation
· Media Preparation
· Serial Dilutions
· 16s rRNA Analysis
· Gel Electrophoresis
· DNA Fingerprinting
· Preparation of DNA Libraries
· HACCP, GMP, GLP, ISO 9001
· Australian Water Quality Guidelines
· Techniques of Hybridization & Sequencing
· Sterilization Methods, Autoclave Utilization
· Polymerase Chain Reactions & Western Blotting
· Spread Plating, Plate Count, Viable Count, MPN
· Immunological Assays (Cellular & Serological Techniques)
· Retrieving & Analyzing Nucleic Acid & Protein Sequences from Databases (i.e. GENBANK)
· Differentiations of Bacterial & Fungal Species Using Rapid Test Kits including API, Carbon Utilization Assay (BIOLOG) & Identification Using Microscope
O’Malley, F., Brown, P.J., Stone, D.J., and Smith, L.J. “The use of phospholipid fatty acid analysis to measure impact of acid rock drainage on microbial communities in sediments” Journal of Microbial Ecology, due for publication April 2004.
Stone, P.J., O’Malley, F., Browne, D.J.M. and Smith, L.J. “Phospholipid fatty acid analysis of the impact of acid drainage on aquatic sediment microbiota.” Microbiology America (ISBN 1324-4272), 22(4): A95, 2001.
Stone, P.J., O’Malley, F., Wilkes, K.L., Browne, D.J.M. and Smith, L.J. “The Finniss River: A Natural Laboratory of Mining Impacts - Past, Present and Future.” The Finniss River Symposium ANSTO E/748: 19-22, 2001.
Stone, L.J., Holden, P.J., O’Malley, F. and Browne, D.J.M. “Bacteria as bio indicators of pollution: characterization of the impact of acid mine drainage on microflora using phospholipid fatty acid analysis.” ABA 2000: Proceedings 15th American Biotechnology Conference, p47, 2000.
Stone, P.J., O’Malley, F., Holden, K.M., Browne, D.J.M., Kennedy, R.A., Wilkes, K.L., and Smith, L.J. “Phospholipid Fatty Acid Analysis as a Measure of Impact of Acid Rock Drainage on Microbial Communities in Sediment and Comparison With Other Measures.” ANSTO C616: Proceedings of the Third Brukunga Workshop: Quantifying the degree of ecological detriment in the Dawesley Creek/Bremer River System, 43-46, 2000.
“Microbial Measures of Impact of Acid Rock Drainage on Waterways”, WAMMO:99 Water Management in Metallurgical Operations, 1999
University of New York
Microbial Communities Characterization (1999–2003)
DNA Fingerprinting (1999)
Paternity Tests (1999)
Southern Blotting (1999)
PCR Analysis (1999)
Gel Electrophoresis (1999)
Enumeration of Yeasts & Moulds in Foods (1999)
New Jersey Community College
Military Officer’s Academy, Israel
Logistic Management & Leadership
St John’s Ambulance
First Aid Certificate
TECHNOLOGY SKILLSET (ADVANCED USER)
MS Suite: Word 2000, Excel 2000, PowerPoint, Outlook, Project, Access; Netscape, Internet Explorer, Composer, Front Page, Adobe Photoshop, Ulead Photoimpact,
Minitab, Canoco for Window, Primer, SPSS, Medline, Cambridge Scientific, Biblioscape5
PC, Macintosh, Windows 95, 98, 2000 & Dos 7